eng Rovedar Journal of Lab Animal Research 2980-9703 2025-02-26 4 1 1 11 10.58803/jlar.v4i1.59 69 Tuekam Kayo Raoul Polycarpe 0 https://orcid.org/0000-0002-2578-4898 Oumar Mahamat 0 https://orcid.org/0000-0001-5170-1913 Dita Tsomelou Gric 0 Kada Sanda Antoine 0 https://orcid.org/0000-0001-5596-2862 Youmbie Djanche Duplex Bonheur 0 https://orcid.org/0009-0004-4974-0322 Department of Zoology, Faculty of Science, University of Bamenda, Cameroon, Africa Introduction: Aframomum pruinosum seeds have been widely used in traditional medicine to treat lung infections as a cytoprotective plant-derived medicine. The present study investigated the anti-inflammatory and antioxidant effects of A. pruinosum in Wistar rats subjected to bleomycin-induced lung injury. Materials and methods: Bleomycin was administered subcutaneously on the first, second, and third day of the study, and other substances (Indomethacin 2 mg/kg and aqueous extract 100, 200, and 400 mg/kg) by oral route through water. Thirty-five Wistar rats (5-week-old) were divided into seven groups of five rats each, normal rats (Group 1) received distilled water (5 mL/kg), and control group of  A. pruinosum aqueous extract (Group 2) to observe its adverse effects, received the highest dose of A. pruinosum (400 mg/kg), the negative group (Group 3) received bleomycin 0.3 mg/kg and distilled water 5 mL/kg, positive group (Group 4) received bleomycin 0.3 mg/kg and indomethacin 2 mg/kg, Group 5 received bleomycin 0.3 mg/kg and aqueous extract of  A. pruinosum 100 mg/kg, Group 6 received bleomycin 0.3 mg/kg and aqueous extract of  A. pruinosum 200mg/kg, and Group 7 received bleomycin 0.3 mg/kg and aqueous extract of A. pruinosum 400 mg/kg.  GraphPad Prism 8.0.1.244 was used to compare data. Results: The body weight of rats (group 3) exposed to bleomycin has decreased compared to the normal group. Groups exposed to bleomycin and treated with indomethacin or aqueous extract have increased body weight compared to the negative group. Co-treatment of bleomycin and aqueous extract of A. pruinosum (400 mg/kg) significantly increased lung weight compared to the negative control. Administration of aqueous extract of A. pruinosum (400 mg/kg) significantly decreased the levels of Interleukin 1 beta, TNF-α, Interleukin 1 beta, and increased Interleukin 10 level compared to the negative control. Co-treatment of bleomycin and aqueous extract significantly alleviated the toxic effects of bleomycin in oxidative parameters, such as superoxide dismutase, catalase, malondialdehyde, and protein, compared to the negative control, and protected the rats against oxidative damage. Conclusion: The aqueous extract of A. pruinosum seeds had anti-inflammatory and antioxidant potential effects, which might be considered an effective therapeutic against lung lesions. https://jlar.rovedar.com/index.php/JLAR/article/view/59 Aframomum pruinosum Antioxidant Bleomycin Inflammation Lung