_1749580405 20250610183325000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Journal of Lab Animal Research J Lab Anim. Res 2980-9703 02 26 2025 4 1 Tuekam Kayo Raoul Polycarpe https://orcid.org/0000-0002-2578-4898 Oumar Mahamat https://orcid.org/0000-0001-5170-1913 Dita Tsomelou Gric Kada Sanda Antoine https://orcid.org/0000-0001-5596-2862 Youmbie Djanche Duplex Bonheur https://orcid.org/0009-0004-4974-0322 Introduction: Aframomum pruinosum seeds have been widely used in traditional medicine to treat lung infections as a cytoprotective plant-derived medicine. The present study investigated the anti-inflammatory and antioxidant effects of A. pruinosum in Wistar rats subjected to bleomycin-induced lung injury. Materials and methods: Bleomycin was administered subcutaneously on the first, second, and third day of the study, and other substances (Indomethacin 2 mg/kg and aqueous extract 100, 200, and 400 mg/kg) by oral route through water. Thirty-five Wistar rats (5-week-old) were divided into seven groups of five rats each, normal rats (Group 1) received distilled water (5 mL/kg), and control group of  A. pruinosum aqueous extract (Group 2) to observe its adverse effects, received the highest dose of A. pruinosum (400 mg/kg), the negative group (Group 3) received bleomycin 0.3 mg/kg and distilled water 5 mL/kg, positive group (Group 4) received bleomycin 0.3 mg/kg and indomethacin 2 mg/kg, Group 5 received bleomycin 0.3 mg/kg and aqueous extract of  A. pruinosum 100 mg/kg, Group 6 received bleomycin 0.3 mg/kg and aqueous extract of  A. pruinosum 200mg/kg, and Group 7 received bleomycin 0.3 mg/kg and aqueous extract of A. pruinosum 400 mg/kg.  GraphPad Prism 8.0.1.244 was used to compare data. Results: The body weight of rats (group 3) exposed to bleomycin has decreased compared to the normal group. Groups exposed to bleomycin and treated with indomethacin or aqueous extract have increased body weight compared to the negative group. Co-treatment of bleomycin and aqueous extract of A. pruinosum (400 mg/kg) significantly increased lung weight compared to the negative control. Administration of aqueous extract of A. pruinosum (400 mg/kg) significantly decreased the levels of Interleukin 1 beta, TNF-α, Interleukin 1 beta, and increased Interleukin 10 level compared to the negative control. Co-treatment of bleomycin and aqueous extract significantly alleviated the toxic effects of bleomycin in oxidative parameters, such as superoxide dismutase, catalase, malondialdehyde, and protein, compared to the negative control, and protected the rats against oxidative damage. Conclusion: The aqueous extract of A. pruinosum seeds had anti-inflammatory and antioxidant potential effects, which might be considered an effective therapeutic against lung lesions. 02 26 2025 1 11 https://creativecommons.org/licenses/by/4.0 10.58803/jlar.v4i1.59 https://jlar.rovedar.com/index.php/JLAR/article/view/59 https://jlar.rovedar.com/index.php/JLAR/article/download/59/103 https://jlar.rovedar.com/index.php/JLAR/article/download/59/103 10.1016/j.bbadis.2012.09.014 10.1177/1753466620926956 10.1155/2016/3501373 10.1177/03009858221082228 10.1101/cshperspect.a016295 10.1016/j.lfs.2005.09.012 10.3174/ajnr.A1940 10.1164/rccm.201106-0965OC 10.1152/ajplung.00240.2013 10.1183/09031936.00196412 10.1586/1744666X.5.1.63 10.1042/BSR20171555 10.1165/rcmb.2007-0299OC 10.1371/journal.pone.0059348 10.1177/1934578X1300800638 10.1080/13880209.2017.1285326 10.1155/2022/7257448 Sonibare MA, Onda EE, Ajayi AM, and Umukoro S. Anti – inflammatory and antioxidant effects of Tetrapleura tetraptera (Schumach & Thonn.) Taub. Fruit extract in carrageenan/Kaolin-induced Acute Monoarthritis in rats. Niger J Pharm. 2017; 13(2): 157-166. Available at: https://www.ajol.info/index.php/njpr/article/view/166264 10.30574/wjarr.2020.8.3.0490 10.1016/0003-2697(79)90738-3 10.1016/0003-2697(72)90132-7 10.1016/S0021-9258(19)45228-9 10.1016/S0021-9258(18)57021-6 Rashmi K, and Shenoy KB. Hepatoprotective studies of aqueous leaf and root extracts of Barringtonia acutangula (L.) Gaertn against ethanol induced hepatic stress in rats. Indian J Tradit Knowl. 2020; 19(1): 152-157. Available at: http://op.niscpr.res.in/index.php/IJTK/article/viewFile/30856/465477386 Chen J, and Stubbe J. Bleomycins: towards better therapeutics. Nat Rev Cancer. 2005; 5(2): 102-112. DOI: 10.1038/nrc1547 10.1074/jbc.272.52.33298 10.1038/bjp.2008.79 10.1074/jbc.271.20.11911 10.1002/ffj.1741 10.1074/jbc.M208521200 10.1038/nrc1547 Ukoha PO, Cemaluk EAC, Nnamdi OL, and Madus P. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. Afr J Pure Appl. 2011; 5(8): 237-244. Available at: https://academicjournals.org/journal/AJPAC/article-full-text-pdf/D96D4481286 10.1155/2021/8871301 10.1155/2021/5581041