Effects of Midazolam-Ketamine Anesthesia on the Haematological and Biochemical Parameters Using Haloperidol or Chlordiazepoxide Premedication in Adult Male Bonnet Macaques (Macaca radiata)
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Abstract
Introduction: It is important to capture wild animals with minimal stress to reduce morbidity and mortality. Oral premedicates have the potential to reduce stress during handling and ease the subsequent administration of anesthetic drugs. This study was conducted to evaluate the hematological and serum biochemical changes associated with anesthesia in male Bonnet Macaques using haloperidol or chlordiazepoxide premedication.
Materials and methods: Twelve adult male Bonnet Macaques aged around 4 to 6 years were randomly allotted to two groups of six each. The duration of the study was five hours. Animals of Group I were administered chlordiazepoxide (10 mg/kg body weight) orally and animals of Group II were administered haloperidol (1 mg/kg body weight) orally four hours before anesthetizing with the intramuscular injection of midazolam (0.1 mg/kg) and ketamine (10 mg/kg). Hematological parameters such as hemoglobin concentration, erythrocyte, total leucocyte count, the volume of packed red cells, granulocyte, monocyte, and lymphocyte count were evaluated. Biochemical parameters such as creatine kinase, aspartate aminotransferase, alanine aminotransferase, cortisol, glucose, calcium, sodium, and potassium were evaluated from the venous blood sample collected at 0th minute and 30th minute after induction of anesthesia.
Results: The results of the current study indicate that in hematological parameters, the volume of packed cells was significantly different at 0th and 30th minute in both groups. The total leucocyte count was significantly different at 0th and 30th minute in Group I and Group Ⅱ, and the monocyte count was significantly different at 0th and 30th minute in Group I. For biochemical parameters, a significant difference was observed in creatine kinase in group II at 0th and 30th minute and cortisol at time 0th between Group I and Group II.
Conclusion: These results highlight the impact of anesthesia protocols on stress responses in Bonnet Macaques. Haloperidol premedication was linked to a greater increase in cortisol and creatine kinase, indicating higher stress and muscle damage compared to chlordiazepoxide.
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References
Fahlman, A. Advances in wildlife immobilisation and anaesthesia clinical and physiological evaluation in selected species. Doctoral Thesis, Swedish University of Agricultural Sciences, Sweden, 2008.
Nielsen L. Chemical immobilization of wild and exotic animals. Ames, Iowa, USA: Iowa Sate University Press; 1999.
Paterson J. Capture myopathy. In: West G, Heard DJ, Caulkett N, editors. Zoo animal and wildlife immobilization and anesthesia. Blackwell Publishing, 2007. p. 115-121. DOI: 10.1002/9780470376478.ch10
Manikandan A. Haemato-biochemical studies in bonnet macaque (Macaca radiata radiata) of 15 cases. J. Entomol. Zool. Stud. 2017; 2(5): 4-24. https://www.entomoljournal.com/archives/2017/vol5issue5/ PartZ/ 5-5-385-839.pdf
Arora BM. Reproduction in wild mammalia and conservation. Association of Indian Zoo and Wild Life Veterinarians, 2002. https://nvli.in/union-catalogue/reproduction-wild-mammalia-and-conservation
Mukherjee P, Roy S, Ghosh D, and Nandi SK. Role of animal models in biomedical research: A review. Laboratory Animal Research. 2022; 38(1): 18. DOI: 10.1186/s42826-022-00128-1
Pierre PJ, Sequeira MK, Corcoran CA, Blevins MW, Gee M, Laudenslager ML, et al. Hematological and serum biochemical indices in healthy bonnet macaques (Macaca radiata). J Med Primatol. 2011; 40(5): 287-293. DOI: 10.1111/j.1600-0684.2011.00466.x
Bonfanti U, Lamparelli D, Colombo P, and Bernardi C. Hematology and serum chemistry parameters in juvenile cynomolgus monkeys (Macaca fascicularis) of Mauritius origin: Comparison between purpose-bred and captured animals. J Med Primatol. 2009; 38: 228-235. DOI: 10.1111/j.1600-0684.2009.00345.x
Buchl SJ, and Howard B. Hematologic and serum biochemical and electrolyte values in clinically normal domestically bred rhesus monkeys (Macaca mulatta) according to age, sex, and gravidity. Lab Animal Sci. 1997; 47: 528-533. PMID: 9355097
Chen Y, Qin S, Ding Y, Wei L, Zhang J, Li H, et al. Reference values of clinical chemistry and hemotology parameters in rhesus monkeys (Macaca mulatta). Xenotransplantation. 2009; 16: 496-501. DOI: 10.1111/j.1399-3089.2009.00554.x
Herndon JG, and Tigges J. Hematologic and blood biochemical variables of captive chimpanzees: Cross-sectional and longitudinal analyses. Comp Med. 2001; 51: 60-69. PMID: 11926304
Mahoney J. Medical care. In: Wolfe-Coote S, editor. The laboratory primate: The handbook of experimental animals. New York: Elsevier-Academic Press; 2005. p. 241- 257. Available at: https://shop.elsevier.com/books/the-laboratory-primate/wolfe-coote/978-0-12-080261-6
Terao K. Management of old world primates. In: Wolfe-Coote S, editor. The laboratory primate: The handbook of experimental animals. New York: Elsevier-Academic Press; 2005. p. 163-73. DOI: 10.1016/B978-012080261-6/50011-8
Venkatesan R, Nagarajan P, Rajaretnam RS, and Majumdar SS. Hematologic and serum biochemical values in aged female bonnet macaques (Macaca radiata) anesthetized with ketamine hydrochloride. J Am Assoc Lab Anim Sci. 2006; 45: 45-48. PMID: 16542043
Ramachandra SG, Ramesh V, Krishnamurthy HN, Ravindranath N, and Shetty KT. Normal hematological and plasma biochemical parameters of the captive bonnet monkey (Macaca radiata). Primates. 1998; 39: 127-134. DOI: 10.1007/BF02557725
Yu W, Hao X, Yang F, Ma J, Zhao Y, Li Y, et al. Hematological and biochemical parameters for Chinese rhesus macaque. PLoS ONE. 2019; 14(9): e0222338. DOI: 10.1371/journal.pone.0222338
Palanivelrajan M, Jayarthangaraj MG, Sridhar R, Parthiban M, and Thirumurugan R. Serum bio-chemical indices in captive Bonnet macaques (Macaca radiata). Indian J Vet Anim Sci Res. 2015; 44(1): 42-45. DOI: 10.56093/ijvasr.v44i1.147720
Koo BS, Lee DH, Kang P, Jeong KJ, Lee S, Kim K, et al. Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride. Lab Anim Res. 2019; 35: 1-6. DOI: 10.1186/s42826-019-0006-0
Venkatesan R, Nagarajan P, Rajaretnam RS, and Majumdar SS. Haematologic and serum biochemical value in aged female Bonnet macaques Macaca radiata anaesthesized with ketamine hydrochloride. J Am Assoc Lab Anim Sci, 2006; 45(2): 45-48. PMID: 16542043
Loomis MR, Henrickson RV, and Anderson JH. Effects of ketamine hydrochloride on the hemogram of Rhesus monkeys (Macaca mulatta). Lab AnimSci. 1980; 30: 851-853. PMID: 7431867
Yoshida T, Suzuki K, Shimizu T, Cho F, andHonjo S. The effects of ketamine anesthesia on hematological and serum biochemical values in female Cynomolgus monkeys (Macacafascicularis). ExpAnim. 1986; 35: 455-461. DOI: 10.1538/expanim1978.35.4_455
Hom GJ, Bach TJ, Carroll D, Forrest MJ, Mariano MA, Traino CE, et al. Comparison of cardiovascular parameters and/or serum chemistry and hematology profiles in conscious and anesthetized Rhesus Monkeys (Macaca mulatta). J Am Assoc Lab AnimSci. 1999; 38: 60-64. PMID: 12086436
CENTRAL ZOO AUTHORITY (Ministry of Environment & Forests, Government of India). Guidelines for Establishment & Scientific Management of Zoos in India 2008. https://cza.nic.in/guidelines-for-management-of-zoos/en
Apfelbach R, Delgado JMR. Social hierarchy in monkeys (Macaca mulatta) modified by chlordiazepoxide hydrochloride. Neuropharmacology. 1974; 13: 11-20. DOI: https://doi.org/10.1016/ 0028-3908(74)90003-3
Heuschele WP. Chlordiazepoxide for calming zoo animals. Int Zoo Yearbook. 1962; 3: 116-119. https://pubmed.ncbi.nlm.nih.gov/ 13907058/
Weiss B, Santelli S. Dyskinesias evoked in monkeys by weekly administration of haloperidol. Science. 1978; 200: 799-801. DOI: 10.1126/science.417399
Weiss B, Santelli S, Lusink G. Movement disorders induced in monkeys by chronic haloperidol treatment. Psychopharmacology. 1977; 53: 289-293. DOI: 10.1007/BF00492366
Chagas JA, Oleskovicz N, de Moraes AN, Flores FN, Corrêa AL, Souza Jr JC, et al. S (+) ketamine and midazolam association by the conventional method of calculation and allometric extrapolation in red howler monkeys (Alouatta guariba clamitans)-clinical and cardiopulmonary response. Ciência Rural. 2010; 40(1): 109-114. DOI: 10.1590/S0103-84782010000100018
Raposo ACS, Ofri R, Schaffer DPH, Gomes Júnior DC, Libório FA, Martins Filho EF, Oriá AP. Evaluation of ophthalmic and hemodynamic parameters in Capuchin Monkeys (Sapajus sp.) submitted to dissociative anesthetic protocols. J Med Primatol. 2015; 44(6): 381-389. DOI: 10.1111/jmp.12200
Furtado MM, Nunes ALV, Intelizano TR, Teixeira RHF, Cortopassi SRG. Comparison of racemic ketamine versus (S) ketamine when combined with midazolam for anesthesia of Callithrix jacchus and Callithrix penicillata. J Zoo Wildl Med. 2010; 41(3): 389-394. DOI: 10.1638/2008-0016.1
Snedcor GW, and Cochran WG. Statistical Methods. 8th ed. Iowa, USA: The Iowa state university press; 1994.
Kim CY, Lee HS, Han SC, Heo JD, Kwon MS, Ha CS, et al. Hematological and serum biochemical values in Cynomolgus monkeys anesthetized with ketamine hydrochloride. J Med Primatol. 2005; 34: 96-100. DOI: 10.1111/j.1600-0684.2005.00097.x
Xie L, Xu F, Liu S, Ji Y, Zhou Q, Wu Q, et al. Age-and sex-based haematological and biochemical parameters for Macaca fascicularis. Plos One. 2013; 8: e64892. DOI: 10.1371/journal.pone.0064892
Lugo‐Roman LA, Rico PJ, Sturdivant R, Burks R, and Settle TL. Effects of serial anesthesia using ketamine or ketamine/medetomidine on hematology and serum biochemistry values in Rhesus Macaques (Macaca mulatta). J Med Primatol. 2010; 39: 41-49. DOI: 10.1111/j.1600-0684.2009.00394.x
Casaco A, Beausoleil I, Gonzalez B, Luaces P, Leon A, Arteaga ME, et al. Hematological, biochemical, respiratory, cardiovascular and electroneurophysiological parameters in African Green monkeys (Cercopithecus aethiopssabaeus). Its use in non‐clinical toxicological studies. J Med Primatol. 2010; 39: 177-186. DOI: 10.1111/j.1600-0684.2010.00410.x
Wu D, Jiang WY, Yang F, Wei SY, Zhou L, Yi Y, et al. Somatometric measurements, and clinical chemistry and hematology parameters in Tibetan macaque (Macaca thibetana). J Med Primatol. 2013; 42: 318-324. DOI: 10.1111/jmp.12062
Wu D, Yi Y, Sun F, Zhou L, Yang F, Wang H, et al. Effects of age and sex on the hematology and blood chemistry of Tibetan Macaques (Macaca thibetana). J Am Assoc Lab Anim Sci. 2014; 53: 12-17. PMID: 24411774
Rovirosa‐Hernandez MJ, García‐Orduña F, Caba M, Canales‐Espinosa D, Hermida‐Lagunes J, and Torres‐Pelayo VR. Blood parameters are little affected by time of sampling after the application of ketamine in Black Howler monkeys (Alouattapigra). J Med Primatol. 2011; 40: 294-299. DOI: 10.1111/j.1600-0684.2011.00474.x
Loomis MR, Henrickson RV, and Anderson JH. Effects of ketamine hydrochloride on the hemogram of Rhesus monkeys (Macaca mulatta). Lab AnimSci. 1980; 30(5): 851-853. PMID: 7431867
Wall HS, Worthamn C, and Else JG. Effects of ketamine anesthesia stress and repeated bleeding on the hematology of vervet monkeys. Lab Anim. 1985; 19: 138-144. DOI: 10.1258/002367785780942633
Bennett JS, Gossett KA, McCarthy MP, andSimpson ED. Effect of ketamine hydrochloride on serum biochemical and hematologic variables in Rhesus monkeys. Vet Clin Pathol. 1992; 21: 15-18. DOI: 10.1111/j.1939-165X.1992.tb00576.x
Larsson MHMA, Birgel EH, Benesi FJ, Birgel Jr EH, Lazaretti P, Fedullo JDL, et al. Hematological values of Cebusapella anesthetized with ketamine. Braz J Vet Res Anim Sci. 1999; 36(3): 131-135. DOI: 10.1590/S1413-95961999000300005
Bolliger AP, Everds NE, Zimmerman KL, Moore DM, Smith SA, and Barnhart KF. Hematology of Laboratory Animals. In: Weiss DJ, Wardrop KJ, editors. Schlam's veterinary hematology. 6th ed.
USA: Blackwell Publishing LTD; 2010. p. 852-887. https://books.google.com/books/about/Schalm_s_Veterinary_Hematology.html?id=lQXtQn593F4C
Mythili MD, Vyas R, Patra SS, Nair SC, Akila G, Sujatha R, et al. Normal hematological indices, blood chemistry and histology and ultrastructure of pancreatic islets in the wild Indian Bonnet monkeys (Macaca radiate radiata). J Med Primatol. 2005; 34: 35-40. DOI: 10.1111/j.1600-0684.2004.00089.x
Palanivelrajan M, Jayathangaraj MG, Sridhar R, and Parthiban M. Haematology of captive Bonnet Macaques (Macaca radiata). Tamilnadu J Vet Anim Sci. 2011; 7: 216-217. https://www.researchgate.net/publication/236297827_HAEMATOLOGY_OF_CAPTIVE_BONNET_MACAQUES_Macaca_radiata
Rahaman H, Srihari K, and Krishnamoorthy RV. Comparative haematology, haemochemistry and electrocardiography of the Slender Loris and Bonnet monkey. Lab Anim. 1975; 9: 69-78. DOI: 10.1258/002367775780994880
Balasubramanyam D, Geetha G, Shanthi G, and Srinivasan, R. Haematological studies in Bonnet Macaque monkey (Macaca radiata). Indian J Field Vet. 2011; 6: 32-33. Available at: https://www.indianjournals.com/ijor.aspx?target=ijor:ijfv&volume=6&issue=4&article=010
Ives M, and Dack GM. Alarm reaction and normal blood pictures in Macaca mulatta. Lab Clin Med. 1956; 47: 723-729. Available at: https://www.cabidigitallibrary.org/doi/full/10.5555/19561404548
Benjamin MM. Outline of veterinary clinical pathology. 3rd ed. New Delhi: Kalyani publications; 2001. https://books.google.com/ books/about/ Outline_of_Veterinary_Clinical_Pathology.html?id= m11q9zqMe2EC
Bentson KL, Capitanio JP, and Mendoza SP. Cortisol responses to immobilization with Telazol or ketamine in baboons (Papio cynocephalus/anubis) and rhesus macaques (Macaca mulatta). J Med Primatol, 2003; 32(3): 148-160. DOI: 10.1034/j.1600-0684.2003.00018.x
Abbott DH, Keverne EB, Bercovitch FB, Shively CA, Mendoza SP, Saltzman W, et al. Are subordinates always stressed? A comparative analysis of rank differences in cortisol levels among primates. Horm Behav. 2003; 43(1): 67-82. DOI: 10.1016/S0018-506X(02)00037-5
Arnemo JM, and Caulkett N. Stress. In: West G, Heard DJ, Caulkett N, editors. Zoo animal and wildlife immobilization and anesthesia. 1st ed. Asia: Blackwell Publishing; 2007. p. 103-110. DOI: 10.1002/9780470376478.ch8
Puri CP, Puri V, and Kumar TA. Serum levels of testosterone, cortisol, prolactin and bioactive luteinizing hormone in adult male rhesus monkeys following cage-restraint or anaesthetizing with ketamine hydrochloride. Eur J Endocrinol. 1981; 97(1): 118-124. DOI: 10.1530/acta.0.0970118
Fuller GB, Hobson WC, Reyes FI, Winter JSD, and Faiman C. Influence of restraint and ketamine anesthesia on adrenal steroids, progesterone, and gonadotropins in rhesus monkeys. Proc Soc Exp Biol Med. 1984; 175(4): 487-490. DOI: 10.3181/00379727-175-41825
Marcus EL, Vass A, and Zislin J. Marked elevation of serum creatine kinase associated with olanzapine therapy. Ann Pharmacother. 1999; 33(6): 697-700. DOI: 10.1345/aph.18282
Meltzer HY, Cola PA, and Parsa M. Marked elevations of serum creatine kinase activity associated with antipsychotic drug treatment. Neuropsychopharmacology, 1996; 15(4): 395-405. DOI: 10.1016/0893-133X(95)00276-J
Montero PFJ, Muñoz AJ, Berlango JA, Durán SM, Calderón DLBVJ, Jiménez ML, and Torres MJ. Increase of blood levels of creatine kinase following intramuscular injection. Med Clin. 1996; 107(17): 649-654. PMID: 9064405
Williams ES, and Throne ET. Exertional myopathy (Capture myopathy). In: Fairbrother A, Locke LN, Hoff GL, editors. Noninfectious diseases of wildlife. 2nd ed. Iowa State University Press; 1996. p. 181-193. https://web.unbc.ca/~wildlifediseaseweb/exertional_myopathy.htm
Spraker TR. Stress and capture myopathy in artiodactyids. In: Fowler ME, editor. Zoo and wild animal medicine current therapy. 3rd ed. Philadelphia: W. B. Saunders Company; 1993. p. 481-488. Available at: https://www.cabidigitallibrary.org/doi/full/10.5555/19952206996