eng Rovedar Publication Journal of Lab Animal Research 2022-12-25 1 1 47 51 23 Narges Lotfalizadeh 0 https://orcid.org/0000-0003-4910-5498 Soheil Sadr 0 https://orcid.org/0000-0002-9068-0673 Pouria Ahmadi Simab 1 https://orcid.org/0000-0003-1507-8815 Ashkan Hajjafari 2 https://orcid.org/0000-0003-2849-9125 Hassan Borji 3 https://orcid.org/0000-0003-4706-4995 Zeynab Bayat 4 https://orcid.org/0000-0002-6865-3003 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran The CRISPR/Cas9 system has been a game-changer in genetics and biotechnology. This study aimed to investigate the existing in vivo uses and their potential to increase our understanding of gene function and biological processes in animal models. With its remarkable precision and accuracy, researchers can now easily edit specific genes within cells and organisms. This technology has opened up new avenues for studying genetic diseases and developing therapies to treat them. One of the most significant advantages of the CRISPR/Cas9 system is its ability to create precise cellular and animal models of human diseases. This allows researchers to investigate the role of genetics in disease development and to develop more effective therapies. For example, the system can correct genetic mutations that cause cystic fibrosis or sickle cell anemia. The therapeutic potential of CRISPR/Cas9 is enormous, especially in gene therapy. By correcting specific genetic mutations, the system can potentially treat human diseases that are currently untreatable with conventional therapies. However, some challenges still need to be addressed before this technology can be used in clinical settings. Despite these challenges, the potential of CRISPR/Cas9 to revolutionize the field of genetics and biotechnology cannot be overstated. Ultimately, this technology has the potential to transform medicine by providing new therapies for a wide range of genetic diseases. https://jlar.rovedar.com/index.php/JLAR/article/view/13 CRISPR Disease models Gene therapy Genome editing In vivo